![]() One of the cleanest and green technologies is just to use water as the solvent. In more recent studies, extraction has advanced to using new and simpler techniques such as microwave-assisted extraction, supercritical fluid extraction, pressurized liquid extraction and ultrasound-assisted extraction. However, these solvents are not only toxic both to humans and the environment they are also expensive especially when carried out at an industrial scale. Various organic solvents with different polarities such as methanol, hexane, chloroform, acetonitrile, benzene and ethyl alcohol has been used over the years to optimize extraction of bioactive compounds. One of the most important step which determines the final outcome of the quality of the herb is the extraction process. The genus Phyllanthus is also a rich source of phenolics and also contains flavonoids, alkaloids, terpenoids and sterols. Currently there is mounting interest in screening plant sources for anti-viral agents. Besides, many therapeutic effects have been reported such as anti-oxidant, anti-inflammatory and analgesic effect. are known to contain high levels of hydrolysable tannins which have been frequently associated with its anti-viral activity. This plant is used to treat urolithiasis, inflammatory bowel disease, diabetes and hepatitis B. niruri, it is also known for its health benefiting properties. Phyllanthus tenellus Roxb., an herbaceous plant belonging to the family of Euphorbiaceae, is closely related to the commonly known Phyllanthus niruri.
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